Once those are compared, it's a matter of looking into what else on the machine may be draining resources, or if behaviour patterns of users might have changed, requiring more resources now than before.
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I have one more to add to Sonja's list, Windows Control Panel Power Options, be sure that is set to High or Maximum Performance and not Balanced, as sometimes in the Balanced setting, the CPUs are not able to hit their turbo frequency thus causing a pretty significant performance hit too.
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