You might want to look at this post:
Have you checked that your Users have synced into the Users list correctly? Your explanation above implies this is not happening. Check the above post to make sure an important setting is checked, re-sync your User Directory Connector, and try again.
Using Active Directory should ensure the user only needs to enter their username and password once, at most.
Hope this helps, and please mark as correct or helpful if it does.
Thanks for the help, I have checked " User access allocation list " , User is appearing in this list and status is "Allocated". Even in Active directory username and password entered correctly ,AD was successfully updated. Still if user other than RootAdmin try to access Hub , user is not able to login using username and Password.
Thanks in advance,