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There are a few things you could verify:
- Make sure that the IIS was installed with Windows Authentication, Basic Authentication, ASP.net and ASP
- Ensure that the QlikView WebServer is not running at the same time
- Browse to the site to check if it is recognized as part of the local Intranet, we have seen instances in which that not being the case resulted in that error
Should none of that help, please file a support ticket and we will take a closer look at it.