Thank you Jacob for reply,
I have already store the certificate in personal certificate store.
Can you explain me more on the statement "user/identity of the apppool running the aspx/asmx site".
It will be great if you share screenshot for the same.
I have attached a screenshot below.
1st certificate is created by Qlik.
2nd certificate is imported by me.
3rd and 4th are service certificate.
I can see you are looking for the certificate called Prachin-7801 is this a renamed version of the client.pfx you have exported from the server?
How does the personal certifacte store look for the user you are running your VS project with (look in the MMC on your dev machine and select current user). This might point you in the right direction.