No. Qlik Sense does not identify if a file is currently in use before attempting to utilize it, what could happen is a reload failure if you get a lock on the file, such as while saving your edits, right when QS is trying to read from it.
Now, this is not very likely since unless the file is particularly large the save times during which the file is locked would be relatively short in most cases. If you wish to mitigate this you can write a retry loop such as we advise for dealing with QVD issues.
During STORE command the File Save occasionally fails
But generally, it just means that the reload task will pick up the modified data when next it runs after you've saved whatever manual changes are needed
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